By J. H. M. Pinkerton (Eds.)
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Extra info for Advances in Oxytocin Research. Proceedings of a Symposium Held on 1st May 1964 Under the Auspices of the Blair–Bell Research Society at the Royal College of Obstetricians and Gynaecologists, Sussex Place, Regent's Park, London, England
Sephadex gel filtration separates oxytocin and substances of similar molecular size on the one hand, from proteins and inorganic salts on the other. It is important to remove the plasma proteins since these interfere with the in vivo assay, especially by shortening the survival period of the assay animal. The presence of moderate or large amounts of salts is undesirable since this 56 ADVANCES I N OXYTOCIN limits the degree to which solutions may be concentrated prior to assay and chromatography.
FIG. 4. Responses of guinea-pig ileum in vitro—top tracing; guinea-pig blood pressure—middle tracing—and (in the same animal) intra-mammary pressure— bottom tracing. Observations were conducted simultaneously for all three records and the time scale is common. At time 0 plasma at 38°C brought into contact with fine glass beads. 0· 3 ml of this plasma added to the in vitro organ bath or given intra-arterially to the intact guinea-pig, at points marked f . Between the 2nd and 10th min, the blood is active in stimulating the ileum and in producing hypotension.
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Advances in Oxytocin Research. Proceedings of a Symposium Held on 1st May 1964 Under the Auspices of the Blair–Bell Research Society at the Royal College of Obstetricians and Gynaecologists, Sussex Place, Regent's Park, London, England by J. H. M. Pinkerton (Eds.)